Journal: Virus Research

Article Title: Epitope mapping of severe acute respiratory syndrome-related coronavirus nucleocapsid protein with a rabbit monoclonal antibody

doi: 10.1016/j.virusres.2021.198445

Figure Lengend Snippet: Recombinant proteins expressed in E.coli.

Article Snippet: HRT-8 cells were infected with 0.01 MOI OC43 coronavirus obtained from ATCC (VR-1558) in 24-well plates and then received the 4-day culture at 33 °C in a humidified 5% CO 2 atmosphere.

Techniques: Recombinant, Plasmid Preparation, Sequencing

Journal: Virus Research

Article Title: Epitope mapping of severe acute respiratory syndrome-related coronavirus nucleocapsid protein with a rabbit monoclonal antibody

doi: 10.1016/j.virusres.2021.198445

Figure Lengend Snippet: Characterization of RAb recognizing human CoV NPs. (A) Coomassie-stained SDS-PAGE of purified NPs of seven human CoVs. (B) and (C) Western blot and ELISA analysis of RAb cross-reacting with NPs of seven human CoVs. M: protein ladder marker. (D) Immunocytochemistry analysis of RAb detecting Ad293 cells transfected with pcDNA-SARS-CoV-2-NP, and HRT-8 cells infected with CoV OC43. Mouse anti-OC43-RBD and anti-PBS sera were used as the controls.

Article Snippet: HRT-8 cells were infected with 0.01 MOI OC43 coronavirus obtained from ATCC (VR-1558) in 24-well plates and then received the 4-day culture at 33 °C in a humidified 5% CO 2 atmosphere.

Techniques: Staining, SDS Page, Purification, Western Blot, Enzyme-linked Immunosorbent Assay, Marker, Immunocytochemistry, Transfection, Infection

Journal: Virus Research

Article Title: Epitope mapping of severe acute respiratory syndrome-related coronavirus nucleocapsid protein with a rabbit monoclonal antibody

doi: 10.1016/j.virusres.2021.198445

Figure Lengend Snippet: Quantification of SARS-CoV-2 NP by double-antibody sandwich ELISA. (A) Double-antibody sandwich ELISA was established. The plates were coated with RAb, then incubated with purified SARS-CoV-2-NP protein under increasing concentrations. Mouse antiserum against SARS-CoV-2-NP and HRP-conjugated secondary antibody was then added in turn. (B) Double-antibody sandwich ELISA was used to quantify SARS-CoV-2-NP expressed in Ad293 cells. Purified SARS-CoV-2-NP (2 μg/mL) was used as the positive control, and Ad293 cellular supernatant was used as the negative control. HAdV-7, RSV, InfA, HCoV-OC43, and HCoV-229E could not be detected by this sandwich ELISA.

Article Snippet: HRT-8 cells were infected with 0.01 MOI OC43 coronavirus obtained from ATCC (VR-1558) in 24-well plates and then received the 4-day culture at 33 °C in a humidified 5% CO 2 atmosphere.

Techniques: Sandwich ELISA, Incubation, Purification, Positive Control, Negative Control